HM57

Clone name: HM57

Specificity: CD79a

Species/Isotype:  Mouse IgG1k

Immunogen: A synthetic peptide, corresponding to amino acids 202-216 from the CD79α protein

Applications:

- Flow Cytometry, (intracellular)

- Western Blot

- Immunoprecipitation

- Immunohistochemistry : fresh and FFPE tissues (is improved by heat mediated antigen retrieval using Citrate pH6.0 or Tris EDTA  pH9.0)

Other

This antibody recognizes CD79a in all mammalian species tested (including Monotremes, Marsupials and Placental) as well as in some birds and reptiles. This is due to the high degree of conservation of the sequence of the immunogen.  

Positive control : any human lymphoid tissue

Publications:

1. Mason DY, Cordell JL, Tse AGD, van Dongen JJM, van Noesel JM, Micklem K, et al. The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.  J Immunol 1991;147:2474-82.

2. Chuang S-S, Li C-Y. Useful panel of antibodies for the classification of acute leukaemia by immunohistochemical methods in bone marrow trephine biopsy specimens.  Am J Clin Pathol 1997;107:410-18.

3. Detection of T and B Cells in Many Animal Species Using Cross-Reactive Anti-Peptide Antibodies'. JonesM, Cordell JL, Beyers AD, Tse,ADG and Mason DY.  J. Immunol 1993 ; 150 (12) ; 5429-5435

4. Hemsley SW; Canfield PJ; Husband AJ.  Immunohistological staining of lymphoid tissue in four Australian marsupial species using species cross-reactive antibodies.  Immunology and Cell Biology, 1995 73(4):321-5.